APPLICATION: CPD is a summation of the total cell output of a culture over time. It can be used to quantify and compare the cell proliferation kinetics form, the cell proliferation rate, and the cell proliferation capacity of cultures. A distinguishing feature of the CPD data of normal vertebrate tissue cell preparations is ending in a plateau indicative of the progression of cultures to containing only non-dividing cells. A new industry standard (ASTM-I F3716-25) is now available for CPD studies.
CPD data are the input for Asymmetrex®’s TORTOISE Test® KSC Counting technology.
A CPD data graph can be generated by calculating the number of cell doublings for a series of consecutive culture intervals and summing the values sequentially.
To generate CPD for a serially passaged culture enter the following data:
USE EXAMPLE: CPD data developed with the same passage schedule can be used to quantify and compare the proliferation properties of any serially passaged cell cultures. However, CPD data are particularly useful for analysis of vertebrate tissue cells in cancer research. When cultured cells are immortalized or neoplastically transformed, the slope of their CPD data increases and the terminal proliferation arrest plateau no longer occurs.
A CPD data graph can be generated by calculating the number of cell doublings for a series of consecutive culture intervals and summing the values sequentially.
Initial Cell Number: